rabbit anti mouse glut 1  (Cell Signaling Technology Inc)

Journal: Journal of Neuroinflammation

Article Title: Scavenger receptor CD36 governs recruitment of myeloid cells to the blood–CSF barrier after stroke in neonatal mice

doi: 10.1186/s12974-022-02388-z

Figure Lengend Snippet: Accumulation of myeloid cells is attenuated in the ipsilateral CP of neonatal CD36 KO mice subjected to tMCAO. A Representative images of CPs at the level of the lateral ventricles immunostained with GLUT-1 (blue) and Iba1 (green) ipsilateral and contralateral to tMCAO in WT and CD36 KO mice at 3 h reperfusion. Scale bar = 50 µm. B Quantification of the number of Iba1 + cells in the ipsilateral and contralateral CPs in WT and CD36 KO mice at 3 h reperfusion . C–F Quantification of CD45 + CD11b + myeloid cells ( C ), CD45 + CD11b + Ly6c hi inflammatory monocytes ( D ), CD45 + 11b + Ly6c int Ly6g + neutrophils ( E ), and CD45 + 11b + Ly6c int Ly6g − patrolling monocytes ( F ) ipsilateral and contralateral to tMCAO in WT and CD36 KO mice at 3 h and 13 h reperfusion. Two-way ANOVA with post-hoc Tukey’s Multiple Comparison test was performed to compare groups with multiple independent variables ( B – F ). Dots represent individual mice. Data are shown as mean ± SD. Individual p values are listed within figures for data that are significant

Article Snippet: Double-immunofluorescence was performed on adjacent sections blocked in 10% NGS/PBST and incubated overnight in 2% NGS/PBST with rabbit anti-mouse GLUT-1 (1:500, AbCAM), rabbit anti-Iba1 (1:500, WAKO), anti-mouse Timp1 (1:200, TFS) followed by appropriate secondary antibodies purchased from Invitrogen and DAPI.

Techniques: